When it comes to highly securing a folder in Windows 7, there are not many good options out of the box. You can use BitLock to secure the whole drive but it only available in Ultimate and Enterprise edition. You can also use EFS to secure specific folders or files but only if you are using Profession edition or up. Both methods requires your folders, files running on a NTFS drive. Here is a tool that covers a much wider use and you may find very useful.
Secure Folder is an easy-to-use free folder security software that lets you hide, lock and encrypt folders using 256-bit AES encryption. It works on all modern Windows system, from XP and up, and folders on various partition formats. NTFS, FAT32, and FAT volumes are all supported.
The option Hide and Lock are utilizing Windows built-in security feature. A simple of change on permissions and attributes can fairly easy to crack the protection. So if you want your data in bullet-proof vault, you need to Encrypt your folder when securing them. The Encrypted files remain in secure even after you moved the data.
After you download and install the program, you need to set up the password along with your email address for the first time using the tool. The email address will be used when password recovery needed later on.
You will need to type in the password every time when using the tool.
The main window lists all folders currently added in program. You can secure or insecure them by easily clicking on the Secure and Insecure button in the toolbar.
There are a few additional useful features added from Tools dropdown menu. You can Hide any existing drive, Private Sweep files to permanently wipe out, and Recover Locked Folder that has been previously locked.
You can also add the Lock/Unlock command right into Context Menu, and/or Send To list. Hiding Security tab from the folder properties is also considered a step to add more difficulty to the crackers.
If those options provided in this tool don’t meet your need, you might want to check out the great alternative TrueCrypt for more options.